Multi-tissue circadian proteome atlas of wild-type and Per1-/-/Per2-/- mice
Brief Method: To generate
double knockout (DKO) mice,
mutant mice were bred with WT C57BL/6J mice for a minimum of 10 generations.
Homozygote mating was employed for the generation of
Per2 DKO mice.
Before collecting tissues, all mice were synchronized for at least 1 week under a cycle
of 12 hours of light and 12 hours of darkness.
Following the synchronization period,
both WT and DKO mice were released into constant darkness.
Tissue samples were collected every two hours for two consecutive days from eight different tissue types,
including the thalamus, SCN-containing region, liver, gallbladder with bile,
brown adipose tissue, kidney, heart (containing both ventricle and atrium),
and gastrocnemius muscle.
In total, 11,651 proteins were quantified across eight types of tissues using TMT-based proteomics.
Then, rhythmic proteins were analyzed by MetaCycle.